For citation purposes: Almelkar SI, Patwardhan AM, Divate SA, Agrawal NB, Bhonde RR, Chaukar AP. Fibrin matrix supports endothelial cell adhesion and migration in culture. OA Biology 2014 Mar 14;2(1):5.

Research study


Fibrin matrix supports endothelial cell adhesion and migration in culture.

S Almelkar, A Patwardhan, S Divate, N Agrawal, R Bhonde, A Chaukar

Authors affiliations

(1) Tissue Culture Lab (Applied Medical Biology) -316, Department C.V.T.S,Seth G.S.Medical College and K.E.M Hospital, Parel,Mumbai-12,MS,INDIA


(3) Department of Pathology, Seth.G.S.Medical College and K..E.M Hospital,Parel,Mumbai 400012, MS,India.

(4) Manipal Institute of Regenerative Medicine,10,Service Road,Domlur Layout,Bangalore 560071,India

* Corresponding author Email:



Fibrin glue (FG) mainly contains fibrin which is formed as a result of physiological blood coagulation. Fibrin plays an important role in arresting bleeding. The sealant fibrin glue is organized by using two components such as fibrinogen and thrombin. The transformation of fibrinogen into fibrin gel involves various enzymatic cascade cleavage and polymerization reactions. Enzymatic cascade reactions are thrombin dependent and involves cleavage of fibrinopeptides A and B from fibrinogen to form fibrin monomers. In the current study we investigated the role of fibrin glue which plays a part in the support of adhesion and expansion of vascular endothelial cells.

Materials and methods

Human saphenous vein endothelial cells (HSVECs) and sheep external jugular vein endothelial cells (SEJVECs) were cultured onto the fibrin glue coated tissue culture plate surfaces. HSVECs & SEJVECs cultured separately onto the fibrin glue coated surface resemble the wound healing type of morphology that is scar (healing) like formation.


The results demonstrate that endothelial cells also play an important role in migration, wound healing and support scar development.


It was concluded that fibrin glue (FG) provides a suitable biological matrix surface for the adhesion and migration of endothelial cells which are also involved in scar like morphology development (wound healing) in the culture.

Licensee OA Publishing London 2014. Creative Commons Attribution License (CC-BY)