For citation purposes: Avantaggiato A, Palmieri A, Carinci F, Pasin M, Bertuzzi GL. Biostimulation and biorevitalization: effects on human skin fibroblasts. Annals of Oral & Maxillofacial Surgery 2013 Mar 01;1(2):11.

Research study

Aesthetic Surgery & Medicine

Biostimulation and biorevitalization: effects on human skin fibroblasts

A Avantaggiato, A Palmieri, F Carinci, M Pasin, GL Bertuzzi

Authors affiliations

(1) Master of Aesthetic Medicine, University Tor Vergata, Rome, Italy

(2) Department of Experimental, Diagnostic and Specialized Medicine, University of Bologna, Bologna, Italy

(3) Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy

* Corresponding author Email:



Aesthetic medicine uses many injective techniques; biostimulation (BS) and biorevitalization (BR) are among these. The term BS indicates stimulation of the anabolic functions of dermal fibroblasts such as replication, protein synthesis and production of extracellular matrix components (ECM). BR instead uses the same injective technique but different medical devices. It is a direct supplementation of hyaluronic acid (HA) alone or added to other molecules (i.e. vitamins). This study discusses BS and BR and their effects on human skin fibroblasts.

Materials and methods

In order to verify the different metabolic effects of BS and BR fibroblast cell cultures, RNA extraction, cDNA synthesis and PCR were performed.


BS and BR produce different metabolic effects in fibroblast cell cultures, thus showing that they are different therapies. For example, neutrophil elastine is activated by BS and to a lesser extent by BR, whereas hyaluronan synthase 1 is activated to a higher extent by BR using the medical device with the lowest HA content. Neutrophil elastase, responsible for the degradation of one of the fibrillar components of ECM, is activated to a lesser extent by BS.


Further experiments using more time points (i.e. not only 24 h of cell cultures but also 12, 48 and 72 h) are necessary to give additional insights on fibroblast behaviour after BS and BR. A better comprehension of fibroblast biology will result in a proper clinical application of BS and BR.

Licensee OA Publishing London 2013. Creative Commons Attribution License (CC-BY)