OA Epidemiology

High Prevalence of Extended-Spectrum Beta-Lactamase-Producing Escherichia Coli in People Living and/or Working on Dutch Broiler Farms

Proceedings of the 2013 annual meeting of the Netherlands Epidemiology Society

Volume 1 Issue S1 Abstract 24


P.M.C. Huijbers, RIVM, Bilthoven; WIAS, Wageningen University, Wageningen, the Netherlands
E.A.M. Graat, WIAS, Wageningen University, Wageningen, the Netherlands
A.H.A.M. van Hoek, RIVM, Bilthoven, the Netherlands
M.G van Santen, RIVM, Bilthoven, the Netherlands
P. Hengeveld, RIVM, Bilthoven, the Netherlands
A. Haenen, RIVM, Bilthoven, the Netherlands
S.C. de Greeff, RIVM, Bilthoven, the Netherlands
L.M. Schouls, RIVM, Bilthoven, the Netherlands
A.W. van de Giessen, RIVM, Bilthoven, the Netherlands
E. van Duijkeren, RIVM, Bilthoven, the Netherlands

Contact with broilers on broiler farms has been suggested as a risk factor for ESBL-carriage in humans. Objectives of this study were to estimate the prevalence of, and identify risk factors for, ESBL-carriage in people living and/or working on conventional and organic Dutch broiler farms.

Broiler cloaca swabs and human rectal swabs were collected from 50 conventional and 8 organic broiler farms on day 21-40 of the production cycle (T1), and individuals (conventional n=141; organic n=27) were asked to complete a questionnaire about risk factors for ESBL-carriage. Organic farms were sampled again on day 68 (T2). ESBL- and  CMY-2-producing E. coli were isolated from samples by selective enrichment and identified by combination disc-diffusion test. Risk factors for ESBL-carriage were analysed using logistic regression.

At T1, positive broiler samples were found on all conventional and seven organic farms. At T2, positive broiler samples were found on all organic farms. On conventional farms, human ESBL-prevalence was 25.5% (12/47; 95%CI13.9-40.4 %), 16.3% (14/86; 95%CI9.2-25.8%), and 37.5% (3/8; 95%CI8.5-75.5%) among farmers, family members, and employees, respectively (P=0.38). Of 27 individuals sampled on organic farms, 2 were ESBL-positive at T1, and 4 were ESBL-positive at T2. Only one individual was positive at both samplings.

ESBLs are found on all broiler farms. On conventional farms there was no statistical difference in ESBL-prevalence between farmers and employees who have daily contact with broilers, and family members not in close contact. For organic farms sample size was too low to draw conclusions on this.

Published: 06 Jun, 2013

Licensee OA Publishing London 2013. Creative Commons Attribution License (CC-BY)